The Relationship among Prolactin and Heat Shock Protein70、IL-22 and IL-10 in the Salivary Gland of Sj(?)gren's Syndrome

Objective The paper was aim to explore the relationship among prolactin(PRL) and heat shock protein 70(HSP70)、interlukin-22(IL-22)、interlukin-10(IL-10) in the salivary gland of Sjogren's syndrome.Methods Immunohistochemical methods was used to detect PRL、HSP70、IL-22 and IL-10 in LSG of pSS、sSS and controls.Correlations among these cytokines and correlations among cytokines and Chisholm's score or clinical manifestations were investigated.Results 1.PRL、HSP70、IL-22、IL-10 were all expressed in the acici、ducts and lymphocytes infiltration area in both SS patients and normal controls.Increased PRL-immunoreactivity was found in infiltrating mononuclear cells、acinar and ductal epithelial cells in pSS and sSS patients compared with normal subjects.The expression of HSP70、IL-22、IL-10 was higher in acinar epithelial cells and infiltrating mononuclear cells in pSS and sSS patients than that in normal subjects,increased IL-10-immunoreactivity was also found in ductal epithelial cells in sSS patients compared with normal subjects.2.A certain positive correlation was found between PRL and HSP70(P<0.05)in LSG of pSS and sSS patients; no signiaficant correlation was found between PRL and IL-22;there was a positive correlation between PRL and IL-10 in LSG of pSS(P<0.01) and sSS(P<0.05) patients. No signiaficant correlation was found between HSP70 and IL-22;A positive correlation was found between HSP70 and IL-10 in LSG of pSS(P<0.01) and sSS(P<0.05) patients;there was also a positive signiaficant correlation between IL-22 and IL-10.3.There was no significant correlation among the expression of PRL、HSP70、IL-22、IL-10 and organ involvement、the expression of anti-SSA and anti-SSB antibody、Chisholm's score neither pSS nor sSS patients.Conclusions 1.The LSG epithelial cells can synthetize PRL、HSP70、IL-22 and IL-10 into the local chronic inflammatory reaction of SS.2.There may be plenty of Th17 cells infiltrate and play an important role in the immunologic derangement in the salivary gland of SS.